Tmem192-rfp-3xha
WebTo isolate the lysosomes, antihemagglutinin (anti-HA) magnetic beads were used to pull down the Tmem192-3xHA–lysosome cells (HA-Lyso cells), as described by Abu-Remaileh et al. 15 Briefly, 1 × 10 7 HA-Lyso HEK-293T cells cultured on 15-cm plates (with or without PPI treatment) were used for each lysosomal immunoprecipitation (LysoIP) assay. WebMedicaid Managed Care Requests for Applications. Please note: the official site for NCDHHS procurements is the North Carolina Interactive Purchasing System. …
Tmem192-rfp-3xha
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Webprk5-TMEM192-3xHA PM mutant. Plasmid #149506. Purpose. Transient expression of human TMEM192. Dileucine motifs LL23-24 and LL28-29 have been mutated to AA. Depositor. David Sabatini. Article. Adelmann et al Nature. 2024 Nov 18. pii: 10.1038/s41586. Insert. TMEM192 (TMEM192 Human) Use. Transient expression WebApr 22, 2024 · LysoTag TMEM192-mRFP-3xHA (TMRHA) was generated by subcloning the cDNA of TMEM192 (Origene) together with monomeric red fluorescent protein (mRFP) and 3× haemagglutinin (HA) tag into the NheI and ...
WebGSC#9 stably expressing the lysosomal protein TMEM192 tagged with either 3xHA (HA-lyso) or 2xFLAG (FLAG-lyso) were mechanically lysed. Lysosomes were immunoprecipitated with anti-HA magnetic beads. These immunopurified lysosome fractions were subsequently analyzed by western-blot and label-free quantification (LFQ) proteomic. WebThe original EGFP was removed, and Tmem192-mRFP-3xHA was inserted at NheI and EcoRI sites into the pLJM1 backbone. Vector type Mammalian Expression, Lentiviral Selectable …
http://www.biodragon.cn/cgkt/98493.html WebJan 3, 2024 · We performed centrifugation at 20,000 × g to generate an organelle-enriched pellet, two-step sucrose density gradient centrifugation, enrichment by …
WebTMEM192 (transmembrane protein 192) is a membrane protein that localizes to lysosomes. [ 1] It has a predominant expression in lung, liver, pancreas and kidney. It exists as a …
WebApr 1, 2024 · Cells with endogenously-tagged TMEM192 and stably expressing FLAG-EEA1 are referred to as 293 EL cells, for E ndo-IP and L yso-IP. These cells were also prepared in a form that has a deletion of the APP gene (293 EL APP -/- ) and the same cells reconstituted with a lentivirus stably expressing a patient mutant form (APP Sw;T700N ) which ... cheltenham racecards saturdayWebproduct name : pLJM1-Tmem192-mRFP-3xHA catalog : 134631 more info or order : Addgene product webpage citations: 2 product information Catalog Number : 134631 … cheltenham racecards tomorrowWebTMEM192-3xHA (HA) served as a loading control. (Right) Densitometric analysis of NPC1 level normalized to TMEM192-3xHA (HA). Data are presented as the mean + s.d. of 3 independent experiments. (I) (Left) Confocal analysis of Proximity Ligation Assay (PLA) between TMEM192-3xHA and NPC1. HA-lyso GSC#9 were treated as described in (H). flickcall for edgeWebFeb 8, 2024 · A synthetic cDNA encoding TMEM192-RFP-3xHA was cloned into the pLJM1 lentiviral vector (Lim et al., 2024). TMEM192-FLAG was cloned also cloned in the pLJM1 vector by amplifying the TMEM192 cDNA using primers to append a DYKDDDK (“FLAG”) peptide to the c-terminus of the resulting protein. flick call netflixWebJan 3, 2024 · We performed centrifugation at 20,000 × g to generate an organelle-enriched pellet, two-step sucrose density gradient centrifugation, enrichment by superparamagnetic iron oxide nanoparticles (SPIONs), and immunoprecipitation using a 3xHA tagged version of the lysosomal membrane protein TMEM192. flick canarias 2 slWebMar 29, 2024 · TMEM192 transmembrane protein 192 [ (human)] Gene ID: 202431, updated on 29-Mar-2024. Summary. Enables protein homodimerization activity. Located in several … cheltenham race card thursday 16th marchWebAgencies submitting a proposal in response to this RFP agree that the materials and submittals are prepared at the agency ’s own expense with the express understanding … flick canarias 2