Web2 dagen geleden · The single cell solution was eventually filtered (20 µm filters) to remove clumps and debris, and put on ice, ready for Fluorescent Activated Cell Sorting (FACS). Each single cell suspension was obtained by pooling 8 retinae (from 4 fish, 2 males and 2 females) of uninjured and light-lesioned Tg(pcna:EGFP);Tg(gfap:mCherry) animals. Web19 feb. 2014 · The mCherry filter cube was a 560/40 nm exciter, 630/75 nm emitter and 585LP dichroic mirror (Chroma, Bellows Falls, VT, USA). A 100× HCX plan fluotar oil immersion objective (Leica Microsystems, Wetzlar, Germany) with a numerical aperture of 1.30 was used. 2.1. Strains and Cell Culture
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Web14 apr. 2024 · c Gq-mCherry (red) is also expressed ... The GCaMP6m fluorescence signals were bandpass filtered (MF525-39, Thorlabs) and collected by a photomultiplier tube (R3896, Hamamatsu). Web28 jul. 2024 · (A) Lateral view of female and male embryos of Dsx1 reporter strain injected with control siRNA and Shep siRNA and observed at 48 h after injection. mCherry fluorescence allowed visualization of Dsx1 expression while GFP fluorescence in the nucleus enabled observation of body structures. hal brown fishing tackle
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WebAddgene pLenti-EF1a-Ngn3ER-PuroR-mCherry Sequencing Result - Sequence Analyzer. Browse. Qiao Zhou. Restoring glucose homeostasis with stomach-derived human in…. pLenti-EF1a-Ngn3ER-PuroR-mChe…. Sequences. Sequencing Result #386851. WebIn general, filter sets designed for Rhodamine / Cy3 will work better with shorter … Web20 jan. 2024 · mCherry is a red fluorescent protein (RFP), classified as a luminescent … halbrundholz toom