Webquickly became the go-to flow cytometry expert. Today, Thomas is still the go-to person for flow cytometry and specializes in optimal panel design. In 2024, she joined Phitonex, a … WebAdd 0.1-10 μg/ml of the primary labeled antibody. Dilutions, if necessary, should be made in FACS buffer. Incubate for at least 30 min at room temperature or 4°C in the dark. This step will require optimization. Wash the cells 3 times by centrifugation at 1500 rpm for 5 minutes and resuspend them in 200 μl to 1ml of ice cold FACS buffer*.
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