WebAug 12, 2024 · Could you check whether your annotation file (GTF) and sequence file (FASTA) share the same sequence names? It is possible that some sequence file keep the chromosome name as "1" instead of "chr1". If this is the case, you should modify your sequence file first. Please let me know if you have any further questions. Thanks. Regards. WebMar 28, 2014 · The second problem is a result of a recent update to the circlize package. You need to change the track margins from their initial settings by adding. circos.par (track.margin=c (0,0)) after circos.trackPlotRegion command and before the links are drawn via the circos.link function in the for loop. Sorry for the problems.
Troubleshooting » nf-core
WebFor example: chr1 11873 14409 uc001aaa.3 0 + 11873 11873 0 3 354,109,1189, 0,739,1347, BEDPE format ¶ We have defined a new file format, the browser extensible data paired-end (BEDPE) format, in order to concisely describe disjoint genome features, such as structural variations or paired-end sequence alignments. WebOct 29, 2024 · 0. Assuming that both of your dataframes are loaded into a database (you will have to setup a database like postgres or sql server), the sql equivalent of: m=merge (res, asign, by = c ("chr", "pos")) Is. select * into m_table from res join asign on res.chr = asign.chr and res.pos = asign.pos. Then you will have a new table: company\u0027s 03
BED File Format - Ensembl
WebDec 21, 2024 · Perhaps with the command: This isn't directly possible. However, there is a workaround. Create a fake line within .simple file with just your gene pair (pretend that this is a block rather than just one gene … WebCan you please provide . I have generated reference.dict and reference.fasta.fai properly and my bed file looks like this (tab-delimited): chr1 69090 70008 OR4F5_1 0 + chr1 367658 368597 OR4F29_1 0 - chr1 621095 622034 OR4F16_1 0 - chr1 861321 861393 SAMD11_1 0 + chr1 865534 865716 SAMD11_2 0 + chr1 866418 866469 SAMD11_3 0 + WebIt says that "chr1_KI270762v1_alt" is not found in the sequence dictionary. I run the command as follows: gatk DepthOfCoverage -R hg38.fasta -I sample.final.bam -O DepthOfCoverage -L hg38.interval_list. The chromosomes are named the same way in all my files (i.e. chr1 chr2...) and 'chr1_KI270762v1_alt' is found in both my .fasta and .dict … company\u0027s 02